Which of the following statements accurately describes GFP's fluorescence under specific conditions?

The correct statement regarding GFP's fluorescence is that it exhibits fluorescence in the presence of UV light. Green Fluorescent Protein (GFP) is naturally occurring and has the property of fluorescence, which means it can emit light in the green spectrum when excited by light, particularly ultraviolet (UV) light. The chromophore within the protein undergoes a conformational change upon excitation by UV light, allowing it to emit its characteristic green fluorescence without the need for additional dyes or complex conditions.

This ability to fluoresce under UV light is essentially what makes GFP invaluable in biological research, as it allows scientists to visualize biological processes in real-time within living organisms, such as animals and plants. The fluorescence does not depend on any external dyes or chemicals, making it a unique and straightforward tool for various applications in cellular and molecular biology.

GFP's fluorescence is indeed stable under physiological conditions, but it can be sensitive to extreme environmental conditions, including temperature variations. Therefore, the assumption that it remains stable at all temperatures is not accurate. Furthermore, GFP can readily be observed in living cells, contradicting any notion that it cannot be used in live-cell imaging. Thus, the key takeaway is that GFP reliably fluoresces when exposed to UV light, which is essential for