Which buffers are identified as having the highest salt content?

The selection of Equilibration Buffer and Binding Buffer as having the highest salt content is accurate because these buffers are specifically designed to facilitate the interaction between the target protein, such as Green Fluorescent Protein (GFP), and the affinity column during the purification process.

In the context of an affinity chromatography setup, the Equilibration Buffer prepares the column by ensuring that the binding conditions are optimal, often featuring a higher salt concentration to stabilize protein interactions. This allows proteins to be effectively bound to the matrix. The Binding Buffer, which is used once the sample is applied to the column, typically maintains a similar high salt concentration to promote maximum binding affinity between the GFP and the immobile ligand, ensuring effective capture of the target protein.

Choosing options with lower salt concentrations would not be suitable during the binding phase, as it could lead to reduced interaction with the column, thus affecting the purification yields. Therefore, the identification of Equilibration Buffer and Binding Buffer as having the highest salt content is critical for achieving efficient protein purification.