What volume of TE buffer is added to the column in Step 10?

The volume of TE buffer added to the column in Step 10 is 750 uL. This specific volume is important as it ensures an adequate buffer exchange, facilitating the elution of proteins from the column while maintaining their stability and functionality. The choice of volume is typically determined based on the column size and the amount of protein being purified, ensuring effective interaction with the buffer to achieve optimal purification results.

Using the appropriate volume of TE buffer is key for proper rinsing and elution of the target protein, in this case, Green Fluorescent Protein (GFP), which helps in achieving a concentrated and pure sample for subsequent analysis or applications. The other volumes do not provide the same level of efficiency in this context, either being too small to effectively wash the column or too large, leading to unnecessary dilution of the protein sample.