What should you do while waiting for the centrifuge to finish its cycle?

Preparing the chromatography column while waiting for the centrifuge to finish its cycle is a productive use of time and ensures that you are ready to proceed with the next steps in the purification process as soon as the centrifuge is done. The chromatography column will be essential for isolating the Green Fluorescent Protein (GFP) from the rest of the cellular components, and having it ready beforehand minimizes downtime and improves efficiency in the lab workflow. This preparation can involve setting up the column, equilibrating it with appropriate buffers, and ensuring that all necessary materials and reagents are at hand. By working on this task, you maintain momentum in your experiment and can achieve better overall results in your GFP purification process.