What should be used to transfer the bacterial culture into the centrifuge tube?

The use of a clean pipette to transfer the bacterial culture into the centrifuge tube is appropriate because it allows for precise and controlled dispensing of liquids, which is crucial in laboratory settings to avoid contamination and ensure accurate measurements. Pipettes are designed for handling small volumes of liquids, ensuring that the transfer is efficient and minimizing the risk of spills or exposure to the environment.

In microbiological techniques, maintaining sterility is extremely important to prevent contamination that could affect the results of experiments. Using a pipette maximizes this sterility compared to other options, as it can be single-use or properly sterilized before use.

Spoons, syringes, and droppers may not provide the same level of precision or sterility required for transferring bacterial cultures, which can lead to inconsistent volumes or contamination of the sample. Thus, choosing a clean pipette is the best practice for such tasks.