What must be done immediately after centrifugation of the microcentrifuge tube?

After centrifugation of the microcentrifuge tube, it is essential to remove the tube from the centrifuge promptly. This action is crucial because leaving the tube in the centrifuge can increase the risk of sample contamination and degradation, especially if the sample contains sensitive biomolecules like proteins or nucleic acids. Additionally, if the tube is not removed in a timely manner, gravitational forces can cause the material in the tube to settle back down, potentially compromising the results of the experiment.

Once the tube is removed, other steps can be taken, such as cooling, filling with buffer, or shaking, but the priority is to handle the tube immediately afterward to maintain sample integrity and ensure accurate results.