What is typically a desired outcome when using lower light exposure in GFP experiments?

Minimized photobleaching is a crucial desired outcome when using lower light exposure in GFP experiments. Photobleaching occurs when the fluorescent molecules in GFP are exposed to intense light for extended periods, causing a loss of their ability to fluoresce. By reducing light exposure, researchers can prolong the lifespan and fluorescent activity of GFP, allowing for longer observation times and more accurate readings of fluorescence. This is particularly important in live-cell imaging and other applications where maintaining the integrity of the fluorescent signal is essential. Consequently, lower light exposure aids in preserving the visual quality of the data collected during the experiment.