What is the total volume of culture transferred to the microcentrifuge tube in Exercise 1?

In the context of the lab exercise, the total volume of culture transferred to the microcentrifuge tube would typically be dictated by the standard protocol used for GFP purification practices and the capacities of the equipment involved. In many experiments, especially those focusing on the expression and purification of proteins like GFP, the volume transferred often falls within a range that allows for comprehensive recovery and analysis of the proteins of interest.

Choosing a total volume of 2 mL suggests that this amount is sufficient to yield a good concentration of the GFP for subsequent purification steps, while still being manageable to handle in a microcentrifuge tube. A volume of 1 mL might not capture the entirety of the volume needed for optimal recovery, while smaller volumes, like 0.5 mL, may not provide enough biomass for significant experimental outcomes. On the other end, a volume of 3 mL might be unnecessarily large, possibly complicating steps later in the purification process.

Thus, 2 mL strikes a balance between adequate sample size and practical handling, making it the most logical choice in this context.