What is the importance of using a buffer during GFP purification?

The use of a buffer during GFP purification is crucial for maintaining the stability of the protein throughout the purification process. Buffers help control the pH and ionic strength of the solution, which is vital because proteins, including GFP, are sensitive to changes in these conditions. If the pH fluctuates or the ionic strength is not appropriate, it could lead to protein denaturation, aggregation, or loss of activity. By providing a stable environment, buffers ensure that the GFP retains its structural integrity and functional properties during purification. This allows for the successful isolation and analysis of the protein, ultimately leading to better experimental outcomes.