What is the function of a lysis buffer?

The function of a lysis buffer is primarily to facilitate the release of proteins from cells. This is achieved through the disruption of cell membranes, allowing the intracellular contents, including proteins, to be released into the solution. Lysis buffers typically contain detergents that break down lipid bilayers, as well as salts and buffers that help maintain the proper pH and ionic conditions for protein stability and solubility during the extraction process.

This role is crucial in biochemical analyses and protein purification procedures, as successful lysis is a prerequisite for isolating and studying proteins effectively. By efficiently breaking open the cells, the lysis buffer ensures that the proteins of interest can be accessed and subsequently purified from the mixture. Additionally, the composition of the lysis buffer can be tailored to optimize the yield of the target proteins while minimizing degradation or loss of functionality.