What is the emission wavelength of GFP fluorescence?

The emission wavelength of Green Fluorescent Protein (GFP) fluorescence is around 509 nm. This characteristic emission comes from the unique chromophore within the protein, which is formed through a post-translational modification involving residues in its structure. Upon excitation by light, the chromophore's electrons become excited and then return to their ground state, emitting light primarily in the green range of the spectrum, which corresponds to the 509 nm wavelength.

This specific emission wavelength is utilized in various applications, such as live-cell imaging and molecular biology, making GFP an important tool in biotechnology and research. Understanding the emission wavelength is crucial for selecting appropriate filters and detectors in experiments that utilize GFP as a fluorescent marker.