What is the aim of optimizing ionic strength during GFP purification?

Optimizing ionic strength during GFP purification is crucial because it directly impacts the solubility of proteins and their tendency to aggregate. Ionic strength influences the electrostatic interactions between proteins and other molecules in solution. When ionic strength is adjusted correctly, it can effectively shield these interactions that often lead to aggregation, thereby allowing proteins to remain soluble. This is particularly important for proteins like GFP, as increased aggregation can lead to lower yields and a less effective purification process. By enhancing solubility and reducing aggregation, the purification process can be more successful, leading to higher-quality protein ready for further study or application.