What is a typical use of size exclusion chromatography in GFP purification?

Size exclusion chromatography (SEC) is primarily utilized to separate proteins based on their size. In the context of GFP purification, the typical use of this technique involves separating GFP from smaller molecules and contaminants.

During SEC, as the sample is applied to a column containing porous beads, larger proteins like GFP move through the column more quickly, while smaller contaminants and molecules get trapped in the pores of the beads, resulting in their elution at a later time. This separation mechanism effectively allows researchers to isolate the desired protein (GFP) from undesired smaller substances, thus improving the purity of the final protein solution.

The importance of this technique lies in its ability to provide a gentle method of purification that does not denature proteins due to the fact that it operates under native conditions without the use of harsh chemicals or extreme conditions.

In conclusion, the suitable answer highlights SEC's role in separating GFP from smaller impurities, which is a fundamental aspect of the purification process.