What does the term "biophysical characterization" entail for GFP?

Biophysical characterization of Green Fluorescent Protein (GFP) includes a comprehensive analysis of its properties, such as structure, stability, and kinetics. This detailed assessment helps researchers understand how GFP behaves under different conditions, how it maintains its fluorescence, and its interactions across various environments. By examining these characteristics, scientists can determine how modifications or different environmental factors might affect the protein's performance in applications like imaging or biosensing.

While measuring fluorescence under UV light, determining optimal pH, and examining interactions with cellular components are all important aspects of studying GFP, they are more specific techniques or conditions rather than encompassing the broad scope of biophysical characterization. This process aims to gain a holistic view of the protein, thereby allowing for a better understanding of its functional and structural properties in various experimental contexts.