The mixture generated from bacterial lysate primarily contains what?

The mixture generated from bacterial lysate primarily contains GFP and bacterial proteins. When bacterial cells are lysed to extract proteins, including Green Fluorescent Protein (GFP), the resulting lysate contains not only the target protein but also various other proteins produced by the bacteria during their growth.

In the context of GFP purification, the lysate will contain the GFP that has been expressed in the bacteria along with other cellular components, such as ribosomal proteins, enzymes, and other cellular proteins. This mixture needs to be processed further to isolate GFP from the bacterial proteins.

It is important to note that the presence of DNA and RNA is generally not a primary focus in this context as the question emphasizes the protein content of the lysate. While DNA and RNA may be present in smaller amounts, they are not the main constituents when discussing protein purification. Thus, the correct answer reflects the reality of what is found in the lysate—both the expressed target protein (GFP) and a range of other bacterial proteins.