In which step of the process are the bacteria resuspended?

In the GFP purification process, the bacteria are resuspended in Step 7. This step typically occurs after cell lysis, which is crucial for releasing the GFP from the bacteria's cellular structure. Resuspension involves taking the cell pellet, which contains the lysed cells and their proteins, and mixing it in a suitable buffer to ensure that the GFP and other proteins are in a homogenous solution. This preparation is essential for the following purification techniques, such as centrifugation or chromatography, allowing the GFP to be isolated effectively based on its properties. Resuspending the bacteria correctly is fundamental for the overall success of the purification process.