How is the effectiveness of GFP purification verified in the lab?

The effectiveness of GFP purification is verified through the observation of GFP fluorescence. This approach is essential because green fluorescent protein emits fluorescence when exposed to specific wavelengths of light, which confirms that the protein has been successfully purified. In a lab setting, purified GFP will glow a distinct green color under ultraviolet or blue light, making it easy to assess the presence and purity of the protein.

This method is beneficial because fluorescence provides a direct indication that the GFP is intact and functional. The intensity of the fluorescence can be correlated with the concentration of GFP, allowing for quantitative assessments as well. Observing GFP fluorescence not only confirms the purification process but also serves as a crucial quality control measure to ensure the protein is active and ready for further applications.