How can a cancer-curing protein be recovered from bacterial cells?

The recovery of a cancer-curing protein from bacterial cells is effectively achieved by isolating a colony and lysing the cells. This process involves growing bacterial cultures that express the desired protein and then breaking open the cells to release the intracellular contents, including the target protein.

Lysing the cells is crucial because it allows the proteins inside the bacterial cells to be extracted for further purification. This method can utilize various techniques, such as mechanical disruption, chemical lysis (using detergents), or enzymatic treatments, all of which are designed to ensure that the protein remains intact and functional for subsequent experiments or therapeutic applications.

The other methods, while having their own utility, do not primarily focus on the efficient recovery of proteins from cells. Freezing and thawing can lead to cell disruption but may not be as effective for protein extraction, and filtering through a membrane is generally used for purification rather than initial recovery after cell lysis. Subjecting cells to high temperatures can denature proteins, rendering them non-functional, and is usually not a reliable method for recovering sensitive proteins like a cancer-curing protein.